It seems kind of official: applying any of the following patches to a Windows Server 2012 R2 domain controller appears to disable SMBv1. Patching a member server does not cause any problem.
2022-12 Security Monthly Quality Rollup for Windows Server 2012 R2 for x64-based Systems (KB5021294)
2022-12 Security Only Quality Update for Windows Server 2012 R2 for x64-based Systems (KB5021296)
2023-01 Security Monthly Quality Rollup for Windows Server 2012 R2 for x64-based Systems (KB5022352)
To be fair, Microsoft did announce (April, 2022) that they would be removing SMBv1 going forward. I did not expect that updates would cause a problem in existing systems, nor am I able to find any relevant information about this in the documentation for the updates.
I created a sandboxed network consisting of a Windows 2012 R2 server (domain controller) and a Windows XP SP3 client (domain member). Both computers are VMs running on Hyper-V.
Create and share a folder on the Windows XP client. Browse to the shared folder from the Windows 2012 R2 server. Works fine.
Install any one of the above listed updates on the Windows 2012 R2 server. Browse to the shared folder from the Windows 2012 R2 server. Fails.
Remove the installed update from the Windows 2012 R2 server. Browse to the shared folder from the Windows 2012 R2 server. Works fine.
I’m not sure of the precise root cause for the failure. I’m curious, but don’t really have the bandwidth to keep digging. Moving forward, I will test any domain controller patches on my sandboxed network before applying them to a production network.
Cheers